Cell Culture Workshop

Pune, Maharashtra, India 1 September 2014 - 7 September 2014

Summary

We have planned a series of 7-Days workshops with practical hands-on training on the basic cell culture techniques; media preparation; genomic DNA isolation, estimation and agarose gel electrophoresis; RNA extraction, cDNA preparation, PCR and agarose gel electrophoresis; protein extraction, estimation, SDS-PAGE and coomassie staining of proteins; freezing and thawing of cells; and MTT Assay for proliferation/cytotoxicity. This is a basic workshop intended for beginners to teach and train them in animal cell culture and molecular techniques using the human/cancer cell lines for various experiments. It will be supported by some theoretical classes on basic cell culture techniques, media preparation, gene/protein expression & interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

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